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Results. PMNs from patients with RAP displayed significantly increased PMA-induced oxygen radical production compared to those from the HC and CP patients. PMN"s from RAP patients also displayed increased phagocytosis compared to those from the CP group.Background. This study examined neutrophil respiratory burst and phagocytic activity in a unique population of patients diagnosed with refractory aggressive periodontitis (RAP).Conclusions. The findings demonstrate a larger receptor independent respiratory burst and higher phagocytotic activity in PMNs derived from patients with RAP, when compared to PMNs derived from successfully treated CP patients and periodontally HC.Method. Peripheral blood PMNs were obtained from 12 RAP patients, 10 patients with successfully treated Chronic Periodontitis (CP) and 13 periodontally healthy controls (HC) and were loaded with dihydrorhodamine 123 (DHR) and stimulated with phorbol 12-myristate 13-acetate (PMA). Phagocytosis via the complement and Fcgamma-receptors was also assessed.
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We examined neutrophil function in a unique population of patients diagnosed with refractory aggressive periodontitis (RAP). Methods: Venous blood was obtained from 12 non-smoking patients who had been diagnosed with RAP, 10 patients with chronic periodontitis who had responded to periodontal therapy (CP), and 13 periodontally healthy controls. Methods: Oral rinse samples and venous blood were obtained from 13 patients diagnosed with refractory periodontitis. After isolation of neutrophils from both samples, dihydrorhodamine was used as a fluorescent probe for phorbol 12‐myristate 13‐acetate–mediated ROS production as assessed through flow by: 5. Bacterial Control of Neutrophil Function. The studies presented above clearly have shown that in patients with severe forms of periodontitis, more neutrophils with a prosurvival phenotype are present. These cells should control and even eliminate bacteria growth, but instead they promote an inflammation by: Treponema denticola, a spirochete abundant in the plaque biofilm of patients with severe periodontal disease, perturbs neutrophil function by modulating appropriate phosphoinositide (PIP) signaling.
The pathogenesis of the chronic periodontal disease is associated with a skewed host inflammatory response to periodontal pathogens, such as Porphyromonas gingivalis, that accounts for the majority of periodontal tissue damage. Neutrophils are the most abundant leukocytes in periodontal pockets and depending on the stage of the disease, also plentiful PMNs are present in the inflamed . The weight of evidence seems to suggest that overt neutrophil defects generally lead to a predisposition to aggressive forms of periodontitis. Impairment in neutrophil function(s) in such cases is frequently genetically (or intrinsically) determined, as discussed further below. Refractory periodontitis population characterized by a Hyperactive oral neutrophil phenotype Guy M. Aboodi et al, Introduction • Hypothesis oral neutrophil hyperactivity is related to periodontal disease severity. Neutrophils play a key role in preserving oral health, since low neutrophil counts as well as deficiency in neutrophil functional responses have been associated with periodontal disease. As mentioned before, neutrophils kill pathogens by phagocytosis, degranulation, or .
Aggressive periodontitis (AgP) is a disease characterized by rapid loss of periodontal tissues affecting systemically healthy individuals under age of 30 years. AgP classified into two categories named localized and generalized aggressive periodontitis. It differs from chronic periodontitis (CP) depending on age of onset of the disease, rate of progression of the disease, structure and. Aggressive periodontitis is a rare form of periodontal disease, which is characterized by rapid attachment loss, bone destruction, non-contributory medical history and family history of the cases(1,2). Early identification of this pathology can help prevent early loss of teeth. It is important to. Refractory periodontitis c. Localized juvenile periodontitis d. Rapidly progressive periodontitis Certain aggressive forms of periodontal disease may cause tooth loss at an early age because: a. Affected teeth must be extracted before the disease process spreads c. Diminished neutrophil function d. Impairment of fibroblast function e. associated with severe abnormalities in the neutrophil function, with early onset of aggressive periodontal disease (1). Refractory periodontitis in a Colombian.